Assocation of genotype and allelotype frequance of interleukine 13 with most viralance gene of staphlococcus aureus

Автор: Al-khafaji R.J., Al-fatlawi B.A.Z., Al-mamory A.A.J.

Статья в выпуске: 31, 2024 года.

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The current study was done to isolation and indentification of the Staphlococcus aureus isolates associated with polycystic ovarian syndrome patients and determine the molecular status for these patients and bacteria. The study included 155 clinical samples, which were collected from November 2022 to June 2023. The samples included blood samples - vaginal swabs. About 75 samples were collected from women with polycystic ovarian syndrome (PCOS) and 30 samples were collected from women with polycystic ovarian disease ( PCOD). They visit Imam Al-Sadiq Hospital and Babylon Teaching Hospital for Maternity and Children in Babylon Governorate. While 50samples were collected from women without polycystic ovaries as a healthy group. All samples were cultured on different media for full bacteriological identification . In addition, blood samples were taken in order to studying molecular status. The results of this study on the polymerase chain reaction revealed a number of genes related to Staphylococcus aureus isolates and some of its virulence factors, including genes (mec A, spa, luks). The study revealed the presence of the mec A gene in 5 out of 38 isolates. And by (13.15 %). While the spa gene was present in 9 out of 38 isolates, with a rate of (23.68 %). While the luks gene was present in 7 out of 38 isolates, with a rate of (18.42 %). while the study included the genes of sick people by detecting some genes, including the study of genetic polymorphisms of the gene for cytokine IL13 in patient samples.

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Interleukine 13, staphlococcus aureus, viralance gene

Короткий адрес: https://sciup.org/148328856

IDR: 148328856 | DOI: 10.18137/cardiometry.2024.31.6371

Список литературы Assocation of genotype and allelotype frequance of interleukine 13 with most viralance gene of staphlococcus aureus

Alhamadani, R . M . I . and Tuwaij, S.S.N. (2020) . Genetic Diversity of Vancomycin – Resistant Staphylococcus aureus Isolated From Different Clinical Specimens in Al-Najaf Province. M.Sc. Genetics Department / Faculty of Biology/ University of Sofia (2013), Ph.D. in Science/Biology/ Microbiology / University of Kufa (2020) .
Al-Sehlawi, Z.S.R. (2012). Occurrence and Characterization of 216Ampc Beta-Lactamases in Klebsiella Pneumoniae Isolated from Najaf Hospitals. Ph.D. Thesis. Faculty of Science, University of Babylon Iraq.
Bailey and Scott’s Diagnostic Microbiology (2017). P. 249, 219-225.
Basanisi, M. G.; La Bella, G.; Nobili, G.; Franconieri, I. and La Salandra, G. (2017). Genotyping of methicillin-resistant Staphylococcus aureus (MRSA) isolated from milk and dairy products in South Italy. Food Microbiology, 62, 141-146.
Benson (2001). Microbial applications laboratory manual in 143 -General microbiology . 8th ed . The McGraw – Hill Companies . PP: 168,257 437-261,434.
Chessbrough, M. (2010). District Laboratory Practice in Tropica Countries, Part-2, New York, USA, Cambridge University,: 184- 186.
Cincione, R. I.; Losavio, F.; Ciolli, F.; Valenzano, A.; Cibelli, G.; Messina, G. and Polito, R. (2021). Effects of Mixed of a Ketogenic Diet in Overweight and References 106.
Forbes, B.E .; Sahm, D.F. and Weissfeld, A.S. (2007). Bailey & Scott’s Diagnostic Microbiology.12 th ed. Mosby Elsevier. Texas, USA.
Foster, T.J. (2017). Antibiotic resistance in Staphylococcus aureus. Current status and future prospects. FEMS Microbiol. Rev. 449_ 430, 007.
Ghorbani Tajandareh, S. and Imani Fooladi, A. A. (2016). Distribution of tsst-1 and mecA Genes in Staphylococcus aureus Isolated From Clinical Specimens. Jundishapur journal of microbiology, 9(3), e29057.
Graham ME, Herbert WG, Song SD, Raman HN, Zhu JE, Gonzalez PE, Walther-António MRS, Tetel MJ. Gut and vaginal microbiomes on steroids: implications for women’s health. Trends Endocrinol Metab. 2021;32(8):554–65.
Itriyeva, K. (2022). The normal menstrual cycle. Current Problems in Pediatric and Adolescent Health Care, 6: 101183.
Jowad,R.M. and Yousif G.M. (2013). Detection of some Virulence Factors and Antibiotic Resistance Genes in Staphylococcus aureus Isolated from Clinical Cases in Al-Diwanyia City. M.S.C. in biology \ College of Science, Al-Qadisiya University.
Kareem, S. M.; Aljubori, S. S. and Ali, M. R. (2020). Novel determination of spa gene diversity and its molecular typing among Staphylococcus aureus Iraqi isolates obtained from different clinical samples. New Microbes and New Infections, 34, 100653.
Katsarou, I.; Paraskevopoulou, N. M.; Papadimitriou- Olivgeris, M.; Giormezis, N.; Militsopoulou, M.; Kolonitsiou, F.; ... and Spiliopoulou, I. (2020). Fatality of Staphylococcus aureus infections in a Greek university hospital: role of inappropriate empiric treatment, methicillin resistance, and toxin genes’ presence. European Journal of Clinical Microbiology & Infectious Diseases, 39(3), 443-450.
Lewis S.M. and B.J Bain. 2001. Dacie and Lewis, Practical Haematology. 9th ed, Churchill Livigstone: 447-477.
MacFaddin, J.F. (2000). Biochemical Tests for Identification of Medical Bacteria. 3rd edition. Lippincott Williams and Wilkins, USA.
Mihm, M.; Gangooly, S. And Muttukrishna, S. (2011). The normal menstrual cycle in women. Animal reproduction science, 124: 229-236.
Neamah, A. J.; Ayyez, H. N.; Klaif, S. F.; Khudhair, Y. I. And Hussain, M. H. (2019). Molecular and phylogenetic study of Staphylococcus aureus isolated from human and cattle of Al-Qadisiyah Governorate, Iraq. Veterinary World, 12 (9), 1378.
Rezashateri, M.; Ahrabi, M. and Salehi, M. (2021). Molecular Analysis of the Presence of pvl, spa, and mecA Genes and Their Correlation with a Range of Antibiotics in Staphylococcus aureus Collected from Burn Patients. Iranian Journal of Medical Microbiology, 15(6), 625-637.
Sambrook, J. and Russell, D.W. (2001). Molecular cloning: a laboratory manual (3rd ed.), Cold Spring Harbor Laboratory Press, Cold Spring. Harbor, NY, USA
Shallcross, L.J. ; Williams, K. ; Hopkins, S. ; Aldridge, R.W.; Johnson, A.M. and Hayward, A.C. (2010). Panton-valentine leukocidin associated staphylococcal disease: a cross-sectional study at a London hospital, England. Clin Microbiol Infect 2010; 16(11):1644–1648. PubMed PMID: 20969671.
Springhouse .(2005). Professional Guide to Diseases. 8th ed, Lippincott Williams and Wilkins.Isban; 1:58255.370.
Tong, S. Y.; Davis, J. S.; Eichenberger, E.; Holland, T. L. and Fowler,J. V. G. (2015). Staphylococcus aureus infections,epidemiology, pathophysiology, clinical manifestations, and Management. Clinical microbiology reviews, 28(3), 603-661.
Yurtsever, S. G.; Aygül, A.; Öztürk, İ.; Nemli, S. A.; Kaya, S. and Ermertcan, Ş. (2020). Investigation of Various Virulence Factors and SCCmec Types in the Health care-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains. European Journal of Therapeutics, 26(2), 113-118.
Zarei Koosha, R.; Mahmoodzadeh Hosseini, H.; Mehdizadeh Aghdam, E.;
Zhang, Y.; Ho, K.; Keaton, J. M.; Hartzel, D. N.; Day, F.; Justice, A. E.; Josyula, N. S.; Pendergrass, S. A.; Davis, L. K. and Edwards, D. R. V. (2020). A genome- wide association study of polycystic ovary syndrome identified from electronic health records. American journal of obstetrics and gynecology, 223: 559. e1-559. e21.

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