Bacterial profiles and phenotypic biomarkers of microbiota isolates in habitat: hazard identification factors

The present work focuses on assessing bacterial profiles of microbiota existing on technological equipment applied in food products manufacturing, objects located inside medical and preventive facilities, and water objects in recreation zones; another goal was to examine phenotypic properties of opportunistic pathogenic bacteria isolates as hazard identification factors within the framework of risk assessment concept. Our research objects were strains of Escherichia, Klebsiella, Enterobacter, Staphylococcus, Pseudomonas, Citrobacter and Serratia families that were detected and extracted due to hygienic monitoring activities performed in 2013-2017. Samples were taken via washing, direct inoculation, membrane filtration, and instrumental aspiration technique. Microbial status was analyzed with cultural and biochemical techniques on nutrient and differential-diagnostic media with subsequent confirmation with polymerase chain reaction (PCR). Phenotypic peculiarities were examined in vitro with conventional biochemical and microbiological techniques in conformity with the requirement fixed in Good Laboratory Practice...