Biological effect of copper oxide nanoparticles synthesized by Saccharomyces boulardii against of multidrug resistant bacteria isolated from diabetic foot infections

Objective: In this study, copper oxide nanoparticles are produced using the probiotic Saccharomyces boulardii in order to assess their biological activity. The biological method of producing nanoparticles is gaining popularity due to its benefits over chemical and physical ways of synthesis in terms of affordability and environmental friendliness. Methods: To biosynthesize CuO NPs, copper sulfate was introduced at a concentration to S. boulardii’s cellfree supernatant. Results: The color change of the reaction mixture from light to dark after 150 rpm incubation, as well as the color change and antibacterial behavior, were indicators of S. bularedii’s biosynthesis of CuO NPs. The characterization completed by UV-visible spectroscopy, Atomic force microscopy, Energy Dispersive Spectroscopy, Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, and X-ray diffraction (AFM). The CuO NP absorption spectra in the reaction mixture’s UV visible spectroscopy were (537.93 nm). The XRD showed that CuO NPs’ crystal size was (14.65 nm). The SEM was provided; the shape was uniform and spherical, and the average size (16.03 nm). EDS was used to analyze the presence of elemental CuO NPs. The CuO NPs’ three-dimensional structure was seen by the AFM, and their average diameter was (41.11 nm). The FTIR spectrum reveals a variety of functional groups that are present at various locations. Gram positive and gram negative bacteria that were isolated from diabetic foot infections were multidrug resistant (MDR), and biosynthesized CuO NPs demonstrated antibacterial action against these bacteria (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus mirabilis). In the form of biofilm using a microtiter plate.