Camp concentrations in cardiac mitochondria and serum in the C57Bl/6 mice under independent melanoma B16/F10 growth versus melanoma B16/F10 growth linked to chronic neurogenic pain
Автор: Kit O.I., Frantsiyants E.M., Bandovkina V.A., Neskubina I.V., Shikhlyarova A.I., Kaplieva I.V., Surikova E.I., Pogorelova Y.A., Cheryarina N.D., Trepitaki L.K., Goroshinskaya I.A., Vaschenko L.N., Shatova Y.S., Kuchkina L.P., Kovalenko V.A., Nemashkalova L.A.
Журнал: Cardiometry @cardiometry
Рубрика: Original research
Статья в выпуске: 22, 2022 года.
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The aim of this research work is to study the cAMP level in the cardiac mitochondria and serum in the C57BL/6 strain mice of both genders under the independent melanoma B16/F10 growth versus the melanoma B16/F10 growth linked to chronic neurogenic pain (CNP). Materials and methods. Mice of strain C57BL/6 (n=336) have been grouped as follows: the intact group of the mice (♂n=21; ♀n=21), the reference group (♂n=21; ♀n=21) with the reproduced CNP model, the comparison group (♂n=63; ♀n=63) to include the mice with melanoma B16/F10, and the main test group (♂n=63; ♀n=63) to cover the mice with the melanoma growth against the CNP background. Upon expiration of 1 week, 2 and 3 weeks of the melanoma growth, in the animals of the above experimental groups the cardiac mitochondria have been isolated with the centrifugation using high-performance refrigerated centrifuge Avanti J-E, BECMAN COULTER, USA. With ELISA Kit (RayBio USA) we have determined cAMP concentrations in serum and in the cardiac mitochondria. Results. CNP has induced a decrease in the cAMP level in the cardiac mitochondria by a factor of 3,6 in the female mice only. In the animals of the comparison group the cAMP level in the heart has been increasing beginning with week 2 of the tumor growth on average by a factor of 4, while in the main test group starting from week 1 of the tumor growth it has been recorded 2-4 times higher and was depleted by the end of the experiment. As to the cAMP concentration in serum, the dynamics thereof has not been found to be in correlation with the cardiac mitochondrial data, and its concentration decrease has been recorded both in the females and the males. Conclusion. So, the changes in the cAMP concentration in the cardiac mitochondria demonstrate their gender-specific feature; the female mice as against the males have responded to an independent impact produced by CNP. As to the main test group, CNP has stimulated an increase in the cAMP level in the cardiac mitochondria 1 week earlier than it is the case with the comparison group, and it has resulted in the full cAMP depletion by the 3rd week of the experiment.
Melanoma в16/ f10, mouse strain c57bl/6, camp, mitochondria, heart, chronic neurogenic pain
Короткий адрес: https://sciup.org/148324642
IDR: 148324642 | DOI: 10.18137/cardiometry.2022.22.6268
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