Insecticide susceptibility and detoxification enzyme activity in Musca domestica L. (Diptera: Muscidae) of field population

The purpose of the study consisted in susceptibility to insecticides and the activity of detoxification enzymes in houseflies of Musca domestica L. natural population from premises of livestock farm of Tyumen Region. The objects of the study were larva and 3-5-days old imagoes of M. domestica of the laboratory strain and the first generation obtained from specimens of the field population. The half-lethal doses of acetamiprid, fipronil, ivermectin, and chlorfenapyr for imagoes were calculated by probit analysis method based on the results of the assessment of intestinal insecticidal activities of these substances by the feeding tests. The results of toxicological experiments on the assessment of the sensitivity of the imagoes of M. domestica to insecticides shower that they were tolerant to acetamiprid. The half-lethal dose of acetamiprid for an imago of natural population was more, than for the individuals of laboratory culture, and the resistance indicator to acetamiprid was equal to 2.5-3.4. Laboratory culture and natural population of M. domestica did not differ on the indicator of average mass of one individual of the stage of larva and imago. There were statistically significant differences between enzyme activities of M. domestica specimens of the laboratory strain and the field population concerning monooxygenases in larvae and adults, glutathione-S-transferase, and hydrolases in adults. In the adults of M. domestica (without sex differentiation) monooxygenase activity was 2.6 times lower, glutathione-S-transferase activity was 1.5 times lower, carboxylesterase activity was 2 times lower, and phosphatase activity was 2.9 times lower of the field population than that in the imagoes of laboratory strain. The activity of the studied enzymes in imagoes of field population did not have statistically significant differences depending on sex.