Cytokine-mediated mechanisms for the correction of aggressive behavior by in vitro modulated immunocompetent cells

Violation of neuroimmune interactions is an important link in the pathogenesis of aggression, including the formation of aggressive behavior. Cytokines, being messengers of intersystem functional connections, play an essential role in this. Previously, we obtained priority data on the possibility of editing patterns of aggressive behavior by immunocompetent cells modulated ex vivo with chlorpromazine. Objective: to study the role of cytokines in editing the behavior of aggressive recipients by in vitro modulated syngeneic immunocompetent cells. It has been shown that the correction of aggressive behavior after transplantation of syngeneic splenocytes modulated in vitro by Aminazin is registered against the background of a change in the content of a number of cytokines in the brain structures pathogenetically significant for aggression towards a decrease in pro-inflammatory cytokines IL-1P, IL-2, IL-6, IFN-γ and an increase in the anti-inflammatory cytokine IL-4, which indicates a decrease in neuroinflammation. At the same time, aggressive recipients also showed modulation of cytokine production by peripheral immunocompetent cells, which manifested itself in a decrease in mitogen-stimulated production of IFN-γ, IL-2, IL-6, and IL-10 by splenocytes. Opposite changes in cytokine levels observed during the formation of aggressive behavior and its relief indicate cytokine-mediated editing the behavioral phenotype of aggressive recipients after transplantation of syngeneic immunocompetent cells pre-cultivated with Aminazin. Visualization of functionally active splenocytes pre-cultivated with chlorpromazine in the parenchyma of not only the spleen, but also the brain of aggressive recipients also suggests a direct effect of the injected immunocompetent cells on the CNS cells.