The effect of lithium salts on the lymphocytes of patients with addictive and depressive disorders in experiments in vitro

In vitro experiments conducted a study of the effect of lithium salts (succinate, fumarate, pyruvate, ascorbate and a reference drug - lithium carbonate) on the modulation of the repertoire of surface lymphocyte receptors in blood samples of patients with alcohol dependence and depressive disorders. Blood samples with lithium salts (final concentration 1.2 mmol/L per lithium ion) were incubated for 24 hours in complete RPMI-1640 medium (control -RPMI-1640 medium). The subpopulation composition of lymphocytes was determined by expression of surface receptors by flow cytometry. The total pool of lymphocytes (CD45+) and their phenotypes were determined: (CD3+ CD19-), (CD3+CD4+), (CD3+CD8+), (CD3-CD16+CD56+), (CD3+CD16+CD56+), (CD3-CD19+), (CD3+HLA-DR+), (CD3-HLA-DR+), (CD3+CD95+). Incubation of blood cells of patients with succinate, fumarate, pyruvate and lithium ascorbate in RPMI medium led to a unidirectional change in the repertoire of surface lymphocyte receptors in comparison with lithium carbonate. All lithium salts did not adversely affect lymphocytes. Previously detected antioxidant, cytoprotective and hemoprotective effects of lithium succinate, fumarate, pyruvate and ascorbate suggest their promise for the development of new pharmacological agents with combined normotimic, neuroprotective and antioxidant effects.