Dominant molecular markers of mutation of high oleic oil in sunflower seeds

Currently, sunflower varieties and hybrids with high content of oleic acid are very promising. Thus, it is necessary to develop and validate metods of molecular-genetic searching of mutation in high oleic oil of sunflower of the Russian breeding that can advance breeding process using marker-associated selection. As a material we used constant lines and F1 hybrids from the genetic collection of the V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK) which are differed by a content of oleic acid on seeds. We evolved genomic DNA from cotyledons of 5-7-days sunflower seedlings using SТАB-buffer. Amplification was done in thermal cycler S1000тм (BioRad, США). We used 11 INDEL, HO-specific fragment и SSR-primers developed by foreign authors. In an experimental way, we selected optimal temperatures for hybridization (annealing) for all the primers combinations with DNA matrix. They were from 50 to 65 °С. Sizes of amplified DNA fragments generally corresponded to the same ones the authors of these markers had...