Pheno-genotypical profile of antibiotic resistance of cultures of microorganisms, isolated from farm animals and technological equipment of livestock farms

The authors conducted studies to determine the phenotypic characteristics and genotypic determinants of antibiotic resistance in microbial cultures isolated from farm animals and technological equipment. The analysis of the phenotypic profile of antibiotic resistance of microorganisms showed a fairly wide range of their response to the third-generation aminoglycosides, semisynthetic penicillins + beta-lactamase inhibitors, fluoroquinolones, I-, II- and III-generation cephalosporins, penicillins, macrolides and tetracyclines. It was found out that 40.4 % of the total number of E. coli cultures tested isolated from farm animal feces were multiresistant. 20.1 % of multidrug-resistant E. coli, 13.4 % of Staphylococcus aureus, and 3.9 % of Staphylococcus epidermidis were isolated from milk. Multidrug-resistant cultures – Staphylococcus aureus (72.2 %) and E. coli (26.6 %) were isolated from the infrastructure of livestock buildings. The analysis of the genetic determinants of antibiotic resistance showed that the blaCTX-M and blaOXA-10 genes were found in the vast majority of isolated cultures of E. coli and Enterobacter cloacae, and the aph (31)-llb genes were found in P. aeruginosa. Also, the Shiga toxin gene stx 2 was discovered in a number of E. coli cultures, which can serve as an important etiopathogenetic factor in the occurrence of infectious pathologies in farm animals.