Enzymatiс activity of bacteria from genus Bacillus during hydrolysis of starch-containing vegetable raw materials

The aim of research was to study the comparative amylolytic activity in different species of bacilli from the genus Bacillus. The objectives of the study were to research the amylolytic activity in Bacillus subtilis, Bacillus cereus, Bacillus licheniformis, as well as to establish the number of spores in these types of microorganisms that exhibit the maximum amylolytic effect. The study was carried out in the research and production laboratory "Biotechnology of feed additives" of the Department of Anatomy, Pathological Anatomy and Surgery of the Institute of Applied Biotechnology and Veterinary Medicine, as well as in the Research and Testing Center (SRIC) of the Krasnoyarsk State Agrarian University. Microorganisms were separated out and identified to species from the material presented by us at the Federal State Unitary Enterprise State Research Institution Genetics (Moscow). Spores of bacilli were obtained in the conditions of the research and production laboratory of the department "Biotechnology of feed additives". Counting the number of spores of Bacillus subtilis, Bacillus cereus; Bacillus licheniformis contained in culture fluids was carried out according to the method of General Pharmacopoeia Monograph.1.7.2.0008.15 "Determination of microbial cells concentration" using a Goryaev counting chamber. The study was carried out with 3-fold repeatability to obtain statistically significant results. The zones of iodine discoloration around the droplets of the culture fluid in different bacilli species had different diameters, which indicate different amylolytic activity of the enzyme produced by Bacillus cereus, Bacillus licheniformis, and Bacillus subtilis. Bacillus subtilis has the highest amylolytic activit; slightly lower amylolytic activity was found in Bacillus licheniformis, and the lowest amylolytic activity was found in Bacillus cereus. We used Bacillus subtilis as an active producer of amylolytic enzyme for enzymatic hydrolysis of starch-containing plant raw materials when obtaining from it a sugar-containing feed additive intended for young farm animals. The number of spores giving the maximum amylolytic effect in Bacillus subtilis, Bacillus licheniformis, and Bacillus cereus was 125,000 spores/ml.