Enzymolysis of raw materials as a factor of intensificationof the process of isolation of phenolic substances of sea buckthorn meal

The aim of the research is to study the process of hydrolysis of ballast substances of defatted sea buckthorn meal by enzyme preparations and their multienzyme compositions to increase the yield of the sum of phenolic substances. The research objectives are to study the content of phenolic substances in sea buckthorn meal fermentolysates obtained by processing CelloLux A, Ultraflo, Ultraflo MAX, Brew Zyme BGX, Pectinex BE 3-L and their multienzyme compositions; to study the effect of multienzyme compositions on the degree of destruction of the cell wall of sea buckthorn meal. Five commercial samples of enzyme preparations with various enzyme activitie have been used, as well as five multienzyme compositions based on them. Physical and chemical indicators are determined by standard methods: mass fraction of moisture - according to State Standard 13979.1-68, mass fraction of ash -according to State Standard 13979.6-69, the presence of metallic impurities and foreign substances - according to State Standard 11246-96, the content of extractive substances - according to State Standard 13979.2-94 , reducing sugars - by the permanganate method according to State Standard 8756.13-87, polyphenolic compounds - according to State Standard R 55488-2013, pectin substances - by the calcium-pectate method according to the guidelines "Pectin: isolation methods and properties" (Averyanova E.V., 2015), microbiological safety indicators - according to State Standard 10444.15-94, State Standard 31747-2012 and State Standard 10444.12-2013. Using the example of defatted sea buckthorn meal, the efficiency of using enzyme preparations of cellulolytic and glucanolytic action, as well as multienzyme compositions with combined cellulolytic and glucanolytic, cellulolytic and pectolytic activities for the extraction of phenolic substances, is shown. It is shown that the used fat-free sea buckthorn meal meets the requirements of regulatory documents in terms of basic physical and chemical and microbiological parameters and can be used for the production of functional food ingredients and pharmaceutical substances. It has been proved that the arising synergistic effect when using multienzyme compositions of enzyme preparations BrewZyme BGX - Ultraflo XL and BrewZyme BGX - Pectinex BE 3-L promotes deeper destruction of the cell wall of sea buckthorn meal particles, as a result of which diffusion processes are enhanced during the release of phenolic substances.