The functional state of cell membranes in patients with urolithiasis

We studied the association between metabolic parameters of urine and blood in relationship with physical and chemical state of cell membranes in patients with urolithiasis. Materials and Methods.Thirty five patients (19 men and 16 women) aged between 23 and 61 years with urolithiasis were evaluated. Microviscosity of cell membranes in patients with urolithiasis and healthy subjects was assessed by fluorescent membrane probes using pyrene as a hydrophobic probe. Level of primary (diene conjugates) and secondary (malondialdehyde) products of lipid per oxidation in blood serum and urine was determined. Results. Acellular urine supernatant in urolithiasis patients had lipid concentration (15,17 ± 4,70 mg per mmol of creatinine) which surpassed normal values (0,89 ± 0,16 mg per mmol of creatinine) 17 times, which reflected the destruction of membranes of renal epithelial cells in urolithiasis. An increased excretion of primary products of lipid peroxidation (diene conjugates) was found...