Justification of use of biodegradable coatings on titanium implants, used for dental prosthetics for dogs

Implantation of any foreign material in the body tissues causes inflammatory-reparative response, which is an expression of protective and compensatory function of the tissue. Implantable biomaterials for medical and veterinary practice, must undergo toxicological and morphological studies. The authors studied the interaction of separate functional and biologically active substances on the functional characteristics of fibroblasts, for the use of bio integratable composite material as a coating on ventplant. The material comprises a functional material - poliazolidinammonium modified by hydrate-ions of halogen, and as a biologically active substance - an aqueous dispersion of submicron aggregates of flavonoids. Human dermal fibroblasts isolated from healthy donor skin, which were provided by plastic surgery clinic of the city of Saratov, served the material for the research. The method of tissue explants was used for getting the cell culture of dermal fibroblasts. The starting material for the work was the normal skin biopsies of adult donors obtained after cosmetic surgery while patients were screened for RW, hepatitis, HIV. During the experiment, the optimal dosages of composite components on fibroblasts, which has no inhibitory effect on these cells, was detected. The analysis of the functional status of dermal fibroblasts cultivated on titanium workpieces coated with a surface of the polymer film and propolis, at various concentrations, showed that the formation of the coating on the surface of a biodegradable implant materials must use the following concentrations of substances: Propolis - 1.25 mg per ml the active substance polymer - no more than 0.0001%. This is confirmed by the absence of damage to cells in culture with the possibility of cell adhesion on the experimental materials.