Functional characteristics of the liver

The aim of the study is to evaluate the effect of copper- and molybdenum-containing compounds on the morphofunctional state of the body of laboratory mice. The study was conducted in the laboratory of the Department of Anatomy and Physiology of the Northern Trans-Urals State Agrarian University. The experiment was conducted on male laboratory mice of the same age. For three weeks, the animals were orally administered solutions of ammonium paramolybdate and copper acetate. The doses of trace elements were 0.0125 (optimal dose for molybdenum) and 5 mg/kg (toxic dose for copper). Based on the actual weight of the animals and daily water consumption, the daily dosage of solutions was calculated: 0.001 g/l for ammonium paramolybdate and 0.07 g/l for copper acetate. The laboratory experiment was designed as follows: all mice were divided into 4 groups of 10 mice each. Group 1 - oral administration of clean drinking water (control group); Group 2 Copper - oral administration of copper acetate solution (concentration 0.07 g/l); Group 3 Molybdenum - oral administration of ammonium paramolybdate solution (concentration 0.001 g/l); Group 4 Molybdenum + Copper - oral administration of a mixture of ammonium paramolybdate solutions (concentration 0.001 g/l) and copper acetate (concentration 0.07 g/l). At the end of the experiment, a biochemical analysis of the blood of the experimental animals was performed, enzyme activity (aspartate aminotransferase (AST) and alanine aminotransferase (ALT)), and urea content were determined. Mice with oral administration of copper compounds had a relatively high urea content in blood samples, and they also had increased activity of aspartate aminotransferase and alanine aminotransferase enzymes compared to the control, which also indicates liver dysfunction. The presence of chemical antagonism between copper and molybdenum is confirmed by the fact that the content of the studied enzymes in the blood of animals in the molybdenum + copper group is significantly reduced. This is especially evident in the values of aspartate aminotransferase and alanine aminotransferase activity, which in animals of this group are reduced almost 5 times compared to the same indicator in animals of the Copper group.