Genetic markers of collagen ITGA2 to evaluate the effectiveness of antiplatelet effect of the drug acetylsalicylic acid

Objective. To study the effectiveness of aspirin in patients with drug polymorphism ITGA2 collagen receptor gene.Materials and methods. We examined 88 patients with coronary artery disease and arterial hypertension, receiving aspirin and cardiomagnil on basic therapy β-blockers, ACE inhibitors and statins.The estimation of platelet aggregation activity by light aggregometry using a two-channel laser analyzer 230LA (BIOLA Ltd, Russia). ITGA2 gene polymorphism was determined using the reagents of DNA- Express ( «Liteh», Russia). Used thermocycler with fluorescence detection in real time (Bio-Rad, USA). Results and discussion. Assessment of the distribution of allelic polymorphism ITGA2 showed the predominance of T-allele. For example, among patients it revealed 30,7 % homozygous 1st alleles (C/C), 22,7 % homozygous 2nd allele (T/T) and 46,6 % heterozygous (C/T). Among healthy - 80 % for homozygotes 1st alleles (C/C), 20 % heterozygous (C/T); homozygotes for the 2nd allele (T/T) have been identified. Thus, the majority (77,3 %) patients in the nucleotide substitution ITGA2 gene has been detected in at least one of the chromosomes. In the control group, in contrast, was not observed by the presence of homozygous T allele and homozygous for the alleles dominated C. Distribution of platelet aggregation type polymorphism showed ITGA2 prevalence among patients with high residual platelet aggregation genotype C/T and T/T. Patients with hypo-aggregation substantially uniform distribution of genotypes C/C and T/T. Normal platelet aggregation parameters were found predominantly in patients with the C/C genotype.Conclusions. These results suggest the prevalence of T allele in patients with increased platelet aggregation