Genome expression induced by specific low-intensity EMF as an effective method for increasing immunity

Imprint

Mikhail Y. Rudenko, Vladimir А. Zernov, Olga К. Voronova, Evgenie Y. Bersenev, Irina A. Berseneva. Genome expression induced by specific low-intensity EMF as an effective method for increasing immunity. Cardiometry; Issue 18; May 2021; p.18-23; DOI: 10.18137/ cardiometry.2021.18.1823; Available from: http://www.cardiometry. net/issues/no18-may-2021/genome-expression

For the purpose of research of the cardiovascular system performance, various electromagnetic phenomena are widely considered. In particular, an ECG is treated as a result from the electrical activity by the myocardial cells. As to our research team, at the first stage of creating the cardiometric theory, we have focused on hemodynamics: our attention has been occupied in developing a fresh model of the human blood flow from the point of view of fluid mechanics [1]. Our mathematical concept of the cardiac cycle phase structure, which determines the cardiovascular system performance, has allowed achieving very good results in diagnostics. We have succeeded in making a number of scientific discoveries which have become the basis for a new science: cardiome-try. But our experience has demanded to make further important steps in research, and we have concentrated our efforts on developing new methods of 18 | Cardiometry | Issue 18. May 2021

Submitted: 20.07.2020; Accepted: 18.08.2020; Published online: 21.11.2020

Figure 1. ECG and its spectrum. The spectrum is composed by 24 harmonics.

therapy to effectively solve the problems revealed by cardiometry.

Following this way, comprehensive studies of the methods and means of therapy have been lunched. At that stage, knowledge in the area of electromagnetic field (EMF) physics has been involved. When analyzing an ECG, the authors hereof have found that upon decomposition of every ECG into individual spectral components identified are 24 harmonics. This pattern is peculiar to all ECG curves (see Figure 1 herein). Further studies conducted by our team have shown that the detected harmonics are produced by the action potentials (AP) generated by the cells.

It has been detected that 24 harmonics form some groups, and there are 5 groups of harmonics in total. This is also certain regularity intrinsic thereto. We suggest that the five groups of harmonics may be linked with the five types of adaptation reactions made by a human organism, which are fundamental for human physiology and medicine and which have been discovered by outstanding Russian scientist Professor L. H. Garkavi (1976) [2]. These adaptation reactions are classified as follows: stress, reaction of training, reaction of calm activation, reaction of elevated activation

Figure 2. The ECG energy is calculated using the power of the spectrum in each of the five groups. Each group corresponds to a specific frequency range. The average value of this range is the resonance frequency of the corresponding adaptation reaction. The power spectrum diagram is shown below the ECG curve.

a) The spectrum power under stress

b) The spectrum power under the reaction of training

and over-activation. So, the next challenging step for us has been to identify the respective criteria for relationship between the above adaptation reaction types and the actual ECG shape variants recorded. Our conceptual philosophy for establishing the criteria has been confirmed, and we have created an absolutely fresh method for diagnostics and therapy. It is well known that H. Selye has defined only the stress reaction, while L. H. Garkavi has described not only stress in more detail, but also discovered four other types of the adaptation reactions, which she has extensively investigated. This is evidence for the fact that the level of the Garkavi’s discoveries is considerably higher than that demonstrated by H. Selye. In fact, the level of L. H. Garkavi's discoveries for medicine is equivalent to the value of the D. I. Mendeleev's discovery of his Periodical Table of Chemical Elements for chemistry. It is obvious that the key to designing a highly effective therapy is an application of the adaptation reactions theory by L.Garkavi. As it’s the case with chemistry which is unimaginable without the Mendeleev’ Periodical Table, so we cannot imagine with reasonable confidence the modern medicine without the scientific discovery made by of L. H. Garkavi.

c) The spectrum power under the reaction of calm activation (the norm)

d) The spectrum power under the reaction of elevated activation (in athletes)

e) The spectrum power under over-activation

Figure 3. Power diagrams of the specific ECG spectrum corresponding to the five types of adaptation reactions

We have assumed that the criteria for a non-in-vasive assessment of the adaptation reactions should be reflected in an ECG shape, since the reactions are based on the energy of metabolism, and the ECG spectrum is its mirror. The energy of any signal is calculated based on its spectrum. In case of an ECG, we have found that each of the five spectrum groups corresponds to a certain frequency range. In this range, one of the harmonics has the largest amplitude (see Figure 2 herein). Under different influences, the

non-invasive technology. The relevant data and the curve are displayed in the upper right corner on the screen (marked with an arrow).

Figure 4. Our device Cardiocode is capable of identifying the adaptation reaction type automatically with the use of our own original

Figure 5. Shape of the cell action potential (AP) depends on the actual biochemical processes in the cell membrane.

harmonics energy in the range changes, but never disappears.

Upon calculating the power of the spectrum in each group, we can construct the respective diagram as illustrated in the lower part in Figure 2 herein. Previously, the type of the adaptation reaction has been determined according to the relevant blood test data, it has been just the percentage of lymphocytes which is a key marker of the actual type of the reaction. Upon completion of our studies to reveal the relationships and correlations between the blood test data and the ECG spectral power energy, which have been conducted together with Prof. A. I. Shikhlyarova at the National Medical Research Center of Oncology, Rostov-on-Don, Russia, we have succeeded in identifying five specific diagrams corresponding to the five types of the adaptation reactions (see Figure 3 herein).

Each adaptation reaction can be described by a complete set of its own specific signs or markers. The higher is the reaction level, the more active and excitable is a human individual in question. The norm cor- responds to the calm activation reaction (see Figure 3b herein). Figure 3 shows that the ECG power curve rises under the over-activation reaction reaching its high frequencies.

Our device Cardiocode is capable of automatically identifying the actual type of the adaptation reaction with the use of our original non-invasive technology and displaying it on the screen in the manner as given in Figure 4 herein.

The diagnostic information value of the AP shape is beyond question. But it was necessary for us to clarify what biophysical process the AP shape depends on. Our studies have shown that the shape of the cell's action potential, and, consequently, of the harmonics, depends on the actual biochemical processes, which take place in the cell membrane (see Figure 5 herein).

The authors hereof have completed their original experimental studies of the action potential recovery in the snail’s neurons. Figure 6 shows the data obtained in their experiments. Professor V. I. Orlov from

a) AP bifurcation. Deterioration of the neuron’s state under the influence made by a chemical factor

c) Normalization of APs upon specific EMF exposure

Figure 6. Normalization of the AP shape in the snail neuron upon specific EMF exposure with the use of the EZh-2 device. Exposure time is 5 minutes.

the Institute of Neurocybernetics, Southern Federal University, Russian Federation, has provided support to us in the above experiments. Upon exposure of the isolated CNS neuron in the grape snail Helix pomatia to some specific EMF, we have recorded the responses by the neuron due to initiation of some EMF mechanisms as assessed according to the parameters of the membrane potential (MP) level and the parameters of the recorded action potentials (APs).

The specific EMF exposure has induced reversible hyperpolarization of the neuron membrane, keeping it at the level of trace hyperpolarization under the EMF action. The restoration of the resting membrane potential is accompanied by an enhanced operation of the Na+ /K+ ATPase, which removes the sodium ions from the intracellular space. In addition, there is an accelerated recovery of the concentration of the calcium ions due to the operation of the 3Na+/1Ca2+ antiporter and Ca2+ATPase.

The specific EMF applied by us increases the rate of rise in the initial phase of the AP firing. This is facilitated by opening of a larger number of the sodium and calcium ion entry channels and, as a consequence, by increasing of the amplitude in the next phase and rising the rate of its repolarization with a steeper decline due to opening of a greater number of the potassium ion channels and a faster exit of the K+ via the respective leakage channels.

The regulation of the functional state of the neuron located in the neural network has been objectively recorded using a non-invasive, contact-free, regulation method. Our analysis has shown that the processes in the neuron are similar to the generation of a transmembrane potential by a cardiomyocyte and that they can be considered as a basic model of the neuronal cellular self-regulation in the phase-structured activity by the cardiovascular system.

Our research work has led to a discovery of a fine tool to control the neuron, the central nervous system and other cellular systems in a human organism. The specific EMF frequencies applied by us as stimulation can be accurately graded by its strength, duration and nature of their effects on living tissue; moreover the specific EMF frequencies applied and the electric current induced thereby are close to the natural mechanisms of the generation and propagation of excitation in living tissues.

It is important that the positive effect produced by our specific EMF therapy on the functional state of

Issue 18. May 2021 | Cardiometry | 21

Table 1

Weight parameters of some organs of the endocrine and immune systems in male rats upon the use of specific EMF exposure with EZh-2 in the early post-immobilization period

Groups Animal No. Body weight (g) thymus weight (mg) adrenal gland weight (mg) coef. thymus/ adrenal glands spleen weight (mg) initial final Main test group 1 230 220 140 60 2.3 1500 4 230 220 145 50 2.9 1200 5 230 250 305 55 5.5 1500 7 250 245 125 60 2.1 1300 8 200 205 155 60 2.6 1400 Reference 2 240 pal 6 215 pal 3 225 195 85 80 1.1 2300 9 215 180 60 70 0.86 800 10 230 190 85 70 1.2 1800 the neuron has been experimentally evidenced. This is manifested in the "relaxing" dynamics of changes in the membrane potential of the neuron resulting in hyperpolarization.

Having clarified the dependence of the influence of biochemical processes in the cell membrane on the AP shape, a new question arises: is it possible to normalize the ECG curve by an EMF stimulation targeted at the performance of cardiomyocytes?

The authors hereof have discovered that a contact-free exposure to the natural broadband carrier signals, modulated by some specific frequencies corresponding to the above types of the adaptation reactions, contributes to a pain relieving effect recorded not only for the myocardium, but also for soft tissues in a human organism. In our more detailed studies on the effects of some specific EMF frequencies with the parameters of the adaptation reactions in animals, we have found that immunity is rapidly and powerfully restored. The quantified assessment shows a 3...5-fold immunity enhancement. The assessment is based on the thymus/adrenal mass ratio. Table 1 herein exhibits that the coefficient characterizing the immune system performance, after the use of the EZh-2 device generating the specific EMF frequencies, on average reaches 3. It is significantly higher than that obtained with medication, and it has absolutely no side effects.

The results obtained by us experimentally have inspired us to carry out further investigations. As a consequence, another fundamental discovery has been made by us that is capable of explaining the phenomenal results upon exposure to the specific EMF with the parameters of adaptation reactions. It has been evidenced that the therapeutic effect lies not only in the bio-resonance recovery of the cell membranes, but also in the activation of DNA, namely, in induc- 22 | Cardiometry | Issue 18. May 2021

ing the genome expression. In particular, due to the latter, the intracellular factor HIF1a is triggered. As noted by Prof. E. I. Mayevsky from the Institute of Cell Biophysics, Russian Academy of Sciences, as a result of transcription of information from HRE genes, synthesis of a number of adaptive proteins is significantly accelerated. As a result, the supply of glucose into the cells increases, glycolysis as the main source of ATP under oxygen deficiency is activated, the synthesis of erythropoietin increases and the vascular growth in the hypoxia-affected tissue regions is initiated. This contributes to the stable activation of the anaerobic and aerobic energy-producing processes, provides for a more complete oxidation of all energy substrates and facilitates washout of under-oxidized metabolites from the tissues. Thus, the mobilization of the HRE genes promotes the long-term favorable effects.

In this connection, we should mention that the Nobel Prize in Physiology or Medicine in 2019 was awarded to Gregg Semenza and Peter Ratcliffe for their study of the erythropoietin gene, including how oxygen regulates the genome expression.

Our studies of the organism responses to the effects of the specific EMF frequencies with the parameters of the adaptation reactions allows us to prove the existence of the criteria for the interrelations between the central nervous system and the cardiovascular system performance. In particular, we have detected that it is possible to regulate some human emotions with the use of some specific EMF frequencies. It turned out that some AP shape abnormalities may be markers of some mental disorders like schizophrenia and autism. It has been found that the main problem in case of these diseases is an abnormally altered shape of the action potential that disables action potential firing by the sympathetic and parasympathetic sys-

Figure 7. Curves of the ECG harmonics recorded in different individuals

tems. And we have discovered that an ECG curve is a portrayal of the APs. The APs fingerprint appearance in the spectrum is strictly controlled by the synchronization time. Resynchronization disables feedbacks. Generally, it is reflected in an inadequate behavior of a human individual. The specific EMF frequencies discovered by us normalize the processes in the cell membranes, the action potential shape by recovering feedback in the central nervous system. Figure 7 depicts the curves of the ECG harmonics recorded in different human individuals. The first spectrum curve is recorded in a normal healthy human subject. The harmonics of low frequencies have their own intrinsic amplitude and width. The second spectrum curve is referred to an athlete, before winning the World Championship: the harmonics show lower amplitude and a larger width: this is typical for the high level of the attention concentration, when am individual is aimed at winning and focused on his/her activities. The third spectrum has been identified in a human subject before his suicide act. It is evident that there are very wide harmonics with very small amplitudes. The behavior of the human subject has been recorded to be inadequate, and as a result, the suicide tragedy has been reported.

The developers of the above device have succeeded in their clinical trials to eliminate the pathological APs in schizophrenics, similarly shaped as shown in Figure 7 herein and normalize the AP shaping in them. Regular exposure to the specific EMF frequencies with the characteristics of the adaptation reactions referred to the physiological norm has made it possible for two human individuals with severe schizophrenia to return to normal life. Upon the specific EMF therapy with our original device, individuals suffering from autism show better adequacy in their behavior

And what about the immune system under COVID-19? The EZh-2 devices, which are now commercially produced, successfully cope with the virus infection problems. The favorable clinical outcomes are presented by us in the current issue of our journal. Those who had regularly used our therapy device EZh-2 several months before the pandemic had negative COVID-19 tests.

The device designed by our research team has been commercially manufacturing for several years in Russia [4]. We have closely analyzed the results of long-term effects made by the device on the human organism: the incidence rate of diseases has been sharply decreased, and many chronic problems have been solved. As to the elderly in this case, they remain very active over the age of 90. Reported are also favorable stable therapy effects on animals produced within the shortest time.

The miracle of the specific EMF frequencies with the characteristics of the adaptation reactions are not surprising for the authors hereof. We are just capable of explaining the mechanisms of their operation and their effects from the point of view of science. We continue our research work. First of all, we are aimed at studying the genome expression and its effect made on the cardiovascular system performance.

The results of the combined use of the diagnostics device Cardiocode and the therapeutic device EZh-2 have made it possible to make a number of discoveries in the field of physiology and medicine. It becomes possible to take a new look at the conventional, generally accepted, paradigms of knowledge, and it is very important that the achievements of this level have already become an integral part of the everyday life of many people. We are sure that cardiometry is already creating new accessible technologies that changes the life of the mankind.