Hydrolysis of starch by thermo-and ph-stable glucoamylase at change physico-chemical factors
Автор: Grigirov V.S., Yakovlev A.N., Yakovleva S.F.
Журнал: Вестник Воронежского государственного университета инженерных технологий @vestnik-vsuet
Рубрика: Биотехнология, бионанотехнология и технология сахаристых продуктов
Статья в выпуске: 4 (58), 2013 года.
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Regularity of the change rate of the enzymatic reactions depending on different temperatures, pH values in the formation and decay of the enzyme-substrate complex was investigated. Found that the kinetics of hydrolysis of starch by the action of heat and pH stable glucoamylases is complex as evidenced by the change in the value Km, which is a measure of the affinity of the enzyme to the substrate, active centers studied glucoamylases and starch undergo conformational changes at pH 4,0-5,0; 4,5-5,5 and a temperature of 60-65 °C.
Короткий адрес: https://sciup.org/14040126
IDR: 14040126