Gramin and its toxicological assessment

The aim of the research was to study the acute and subchronic toxicity of the drug gramine in white mice. The micromycete Drechslera graminea served as the basis for the creation of the gramine preparation. Experiments to determine the acute toxicity of the preparation gramine were carried out on outbred white mice (males) weighing 18-20 grams. The drug was administered to animals intraperitoneally, once in doses from 400 to 2400 mg/kg. 6 mice were used for each dose. The condition of the animals was monitored for 14 days. The dose causing the death of 50 % of the animals (LD50) and the absolutely lethal dose (LD100) were determined. LD50 was calculated by the Kerber method. The study of subchronic toxicity of gramine was carried out for 30 days. The drug was administered to 10 mice intramuscularly daily, in the first 4 days, gramine was injected at a dose of 0.1 LD50, and then every 4 days the dose was increased 1.5 times and finally it was 1520 mg/kg. To determine the cumulative properties of the drug in white mice, a "subchronic toxicity" test was used. The reliability of the results obtained was taken into account in accordance with the Student's t-criterion at P function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }