Characteristic of RF-lines from sunflower collection of VNIIMK using microsatellite DNA-markers

Sunflower is one of the most profitable oil crops. The task of sunflower breeding is development of highly productive hybrids. The genetic diversity of initial germplasm is very important in this case. Molecular-genetic markers, particularly microsatellites, are used for the estimation of such material. The purpose of this research concluded in the genotyping of an Rf-lines collection, analyzing its genetic diversity using microsatellite markers. The analysis of 54 Rf-lines from a collection of V.S. Pustovoit All-Russian Research Institute of Oil Crops was carried out using 12 SSR-markers. Genetic passports of all studied lines were composed by these markers, the main indicators of SSR-loci information capacity were determined. The collection is characterized with a high genetic diversity, in total (Nа) 55 alleles were obtained, in average 4.58 alleles per locus. An effective allele amount (Ne) averaged 2.48. An index of polymorphic information content (PIC) was 0.51. A level of line heterogeneity was quite low (5.5%) that certifies their good genetic uniformity. Due to the results, the collection was divided in two cluster at a joint level of 15.8. A minimal genetic distance between lines was equal 0. Eleven lines formed one cluster. The most lines (43 pcs) formed another cluster. The uniqueness of the collection was 91%.