Chromatographic methods determination of zincbatracine antibiotic

Based on the conducted studies using HPLC methods, using diode-matrix, ultraviolet and fluorescent detectors, the composition of zincbacitracin was analyzed. It has been proposed to use for effective extraction of antibiotic from the feed an effective intake of sample preparation on the basis of acetonitrile / methanol / aqueous solution КН2РО4 (0,05М, рН=6,0) (15:45:40 v/v/v), as well as for solid-phase extraction, the Oasis HLB cartridge was selected with a polymer sorbent content that simultaneously possesses hydrophilicity and lipophilicity properties. A method for identifying zincbacitracin by reversed-phase HPLC with pre-columnar derivatization with orthophthalic dialdehyde was developed to enhance fluorescence. Based on the range of detectable contents, this technique will allow to determine the trace amounts of antibiotic at the level of permissible differentiated standards of full-feed mixed fodders, premixes, protein-vitamin supplements, and whole milk substitutes.