Identification of soybean (Glycine max L.) cultivars using microsatellite DNA loci

Intravarietal polymorphism of soybean cultivars was studied. It was resulted two cultivars Lada and Vilana are heterogeneous. Totally 23 soybean cultivars of VNIIMK’s breeding were analyzed on nine known microsatellite loci (SSR). DNA amplification resulted that eight from nine studied loci appeared to be polyallelic, and one locus - Soygy 2 is monomorphic. An average number of alleles per locus was 3.2; locus Satt 9 was revealed to have maximal allele numbers -five. Using system of nine SSR-markers, soybean cultivars were identified, their molecular-genetic passports were done and uniqueness of each cultivar was proved.