In vitro antidiabetic activity of ethanolic extract of Amaranthus viridis linn
Автор: Gunasekaran Sh., Nayagam A.A.J., Gnanasekaran J., Kesavan K., Govindasamy H.
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 4 т.20, 2024 года.
Бесплатный доступ
Objective: To investigate the antidiabetic potential of ethanoilc extract of Amaranthus viridis Linn. in vitro assays. Methods: Ethanolic extract of aerial parts of Amaranthus viridis Linn. was extracted and tested for presence of phytochemical constituents, a-amylase inhibitory activity, determination of glucose adsorption and inhibition of glucose diffusion using dialysis membrane done by plant extracts. Results: Presence of Flavonoids, alkaloids, sugars, quinones, tannins, terpenes, coumarin and phenols.were identified in the extract. The results of in vitro antidiabetic assays showed moderate activity of a-amylase inhibitory activity with increase in the percentage inhibition of alpha amylase enzyme in a dose dependent manner. The IC50 value was found to be 155.60 ^g. The adsorption of glucose by the plant extract was increased remarkably with an increase in glucose concentration. The rate of glucose diffusion was found to increase with time from 30 -180 min, and the plant extract demonstrated significant inhibitory effects on movement of glucose into external solution across the dialysis membrane and compared to control. Conclusion: The antidiabetic effect of aqueous extract of Amaranthus viridis Linn. is mediated by increasing glucose adsorption, decreasing glucose diffusion rate and moderately inhibiting the alpha amylase enzyme thereby exhibiting the potential antidiabetic effect revealed by in vitro models. These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.
Diabetes mellitus, alpha amylase, glucose adsorption, gdri
Короткий адрес: https://sciup.org/143183430
IDR: 143183430
Список литературы In vitro antidiabetic activity of ethanolic extract of Amaranthus viridis linn
- Amin, I. M. (2011). Hypoglycemic effects in response to Abelmoshus esculentus treatment: a research framework using STZ-induced diabetic Rats. International Journal of Bioscience, Biochemistry and Bioinformatics, 1(1), 63.
- Anbu, N., Musthafa, M., & Velpandian, V. (2012). Anti-diabetic activity of polyherbal formulation Aavaraiyathi churnam in alloxan induced diabetic rats. International Journal of Toxicological and Pharmacological Research, 4(4), 77-80.
- Bhutkar, M. A., & Bhise, S. B. (2012). In vitro assay of alpha amylase inhibitory activity of some indigenous plants. Int. J. Chem. Sci, 10(1), 457-462.
- Brindha, P. (1981). Sasikala and Bhimarao, Pharmacognostic studies on Coleus Aromatic Benth. Indian Berage, BMEBR, 12, 17-31.
- Chau, C. F., Chen, C. H., & Lin, C. Y. (2004). Insoluble fiber-rich fractions derived from Averrhoa carambola: hypoglycemic effects determined by in vitro methods. LWT-Food Science and Technology, 37(3), 331-335.
- Dineshkumar, B., Mitra, A., & Manjunatha, M. (2010). A comparative study of alpha amylase inhibitory activities of common anti-diabetic plants at Kharagpur 1 block. International Journal of Green Pharmacy (IJGP), 4(2).
- Gamble, J. S. (1997). Flora of presidency of Madras, botanical survey of India. Vol II. 1163-1164.
- Krishnamurthy, G., Lakshman, K., Pruthvi, N., & Chandrika, P. U. (2011). Antihyperglycemic and hypolipidemic activity of methanolic extract of Amaranthus viridis leaves in experimental diabetes. Indian journal of pharmacology, 43(4), 450-454.
- Kumar, B. S. A., Lakshman, K., Jayaveera, K. K. N., Shekar, D. S., Muragan, C. S. V., & Manoj, B. (2009). Antinociceptive and antipyretic activities of Amaranthus viridis Linn. in different experimental models. Avicenna journal of medical biotechnology, 1(3), 167.
- López, G., Ros, G., Rincón, F., Periago, M. J., Martinez, M. C., & Ortuno, J. (1996). Relationship between physical and hydration properties of soluble and insoluble fiber of artichoke. Journal of agricultural and food chemistry, 44(9), 2773-2778.
- Macharla, S. P., Venkateshwarlu Goli, V. G., Bhasker, K. V., Devi, P. S., Dhanalakshmi, C., & Sanjusha, C. (2011). Effects of anti-inflammatory activity of Amaranthus viridis Linn. Ann. Biol. Res., 2:435-438.
- Narkhede, M. B., Ajimire, P. V., Wagh, A. E., Mohan, M., & Shivashanmugam, A. T. (2011). In vitro antidiabetic activity of Caesalpina digyna (R.) methanol root extract. Asian Journal of Plant Science and Research, 1(2), 101-106.
- Ou, S., Gao, K., & Li, Y. (1999). An in vitro study of wheat bran binding capacity for Hg, Cd, and Pb. Journal of Agricultural and Food Chemistry, 47(11), 4714-4717.
- Ou, S., Kwok, K. C., Li, Y., & Fu, L. (2001). In vitro study of possible role of dietary fiber in lowering postprandial serum glucose. Journal of agricultural and food chemistry, 49(2), 1026-1029.
- Pandhare, R., Balakrishnan, S., Mohite, P., & Khanage, S. (2012). Antidiabetic and antihyperlipidaemic potential of Amaranthus viridis (L.) Merr. in streptozotocin induced diabetic rats. Asian Pacific Journal of Tropical Disease, 2, S180-S185.
- Pareek, H., Sharma, S., Khajja, B. S., Jain, K., & Jain, G. C. (2009). Evaluation of hypoglycemic and anti-hyperglycemic potential of Tridax procumbens (Linn.). BMC complementary and alternative medicine, 9, 1-7.
- Sayeed, M. A., Ali, L., Hussain, M. Z., Rumi, M. A., Banu, A., & Azad Khan, A. K. (1997). Effect of socioeconomic risk factors on the difference in prevalence of diabetes between rural and urban populations in Bangladesh. Diabetes care, 20(4), 551-555.
- Sharma, P., Dubey, G., & Kaushik, S. (2011). Chemical and medico-biological profile of Cyamopsis tetragonoloba (L) Taub: an overview. Journal of Applied Pharmaceutical Science, 1(2), 32-37.
- Shobana, G., Ganga Devi, A., Keerthana, K., Agnel Arul John, N., Jothi, G., Hariharan, G., (2018). Antidiabetic Potential of Polyherbal formulation on Alloxan induced diabetic rats. International Research Journal of Pharmacy. 9, 90-94.
- Singh, L. W. (2011). Traditional medicinal plants of Manipur as anti-diabetics. J Med Plant Res, 5(5), 677-687.
