Indication of caprine arthritis encephalitis virus in RT-PCR and the search for genetic markers

The aim of the study is to monitor goat-breeding farms of the Republic of Tatarstan by CAEV, as well as to search for universal genome loci that are available for all types of CAEV, for use as genetic markers for PCR virus indications. Reliable amplification of the CAEV genome requires the use of marker sequences characterized by maximum specificity and the number of detected strains and isolates of the virus. The marker locus defined in this work allows gene indication of the maximum number of CAEV isolates with a high degree of specificity, confirmed in nBLAST analysis. As a result of PCR analysis to indicate goat arthritis-encephalitis virus, proviral DNA was found in 8 samples, which indicates the possibility of the presence of the virus in this farm.