Induction of callusogenesis in culture of isolated organs of Origanum vulgare L

The influence of hormonal composition of the culture medium, the time of explants introduction in culture in vitro and the type of explants (leaf, stem, petiole) on the induction of callus formation in culture of isolated organs of oregano (Origanum vulgare L.) were investigated. The optimal regime of obtaining aseptic culture from seeds, segments of leaf, stem and petiole have been chosen - sequential processing of 70 % ethanol (1 min) and 50 % “Bradofen” (4 min.) It was shown that the maximum frequency of callus formation (up to 85 %) was observed when using Mu- rashige and Skoog medium supplemented with 1,0 mg/l 2.4-D and 0.5 mg/l 6-BAP. On most tested media the frequency of callus formation was higher by culturing petiole segments compared to leaf and stem explants. It was established that when explants placed on the culture media in spring and winter the frequency of callus formation was 2-4 times larger compared to summer.