Innovative biotechnology of hydrolyzed biomass - peptide ultralysate using selective bacteria

The biotechnological aspects of the production of hydrolyzed biomass of Peptide ultralysates using selective bacteria are considered: Bifidobacterium adolescentis; Bifidobacterium longum; Bifidobacterium breve; Bifidobacterium bifidum; Bifidobacterium animalis; Lactobacillus plantarum; Lactobacillus salivarius; Lactobacillus rhamnosus; Lactobacillus casei; Lactobacillus helveticus; Lactococcus lactis; Lactobacillus delbrueckii subsp. Lactis; Lactobacillus acidophilus; Lactobacillus delbrueckii subsp. Bulgaricus; Streptococcus thermophiles; Propionibacterium freudenreichii; Leuconostoc mesenteroides subsp. dextranicum. Bacterial cultures were obtained in lyophilized form from the All-Russian collection of Industrial microorganisms. Biotechnology includes: primary generation of bacteria with cultivation of microorganisms in a thermostat; preparation of inoculate, with the addition of the first generation; preparation of bacterial biomass, which should be stored for no more than 7 days at 4-10 °C, the number of bacteria should be at least 1•109 CFU/ml; preparation of liquid lysate in a reactor with the addition of an enzyme and separation of biomass, the product should be stored for no more than 4 days at a temperature of 4 °C. After adding a protective medium to the bacterial biomass, the product is sent to spray drying with controlled temperature parameters. The finished product is packed in bags made of aluminum foil and polyethylene, stored (dry form) for 3 years at 25 °C and humidity no more than 60 %. “Peptide ultralysate” consists of products of the physico-enzymatic cleavage of probiotic cells, is a metabiotic, can be used in the production of food, cosmetic products, as well as in technologies with aggressive processing modes.