Using hydroxylated fullerene C60(OH)20-24 to improve bio-yogurt formulation and technology

The paper presents improved formulations and technologies of bioygurt using hydroxylated (water-soluble) fullerene C60(OH)20-24. Compiled formulation of prototypes of bioyoghurt 3.2 % fat content with its introduction into the milk mixture at the stage of normalization (0.04 and 0.08 % by weight of the mixture). Fullerene was produced using biological synthesis at the L.V. Kirensky Institute of Physics SB RAS in the Laboratory of analytical methods for the study of matter. To obtain water-soluble fullerenes, fullerene-containing soot was synthesized in an installation of the kilohertz frequency range at atmospheric pressure. The synthesis of hydroxylated (soluble) fullerene was carried out using nitric acid followed by hydrolysis of polynitrointermediates with water. Milk - raw material for the production of biogurt was used from the zoological farm of the Institute of Applied Biotechnology and Veterinary Medicine of the Krasnoyarsk State Agrarian University. The quality indicators of raw cow milk and finished product were determined by standardized methods operating in the territory of the Russian Federation. The use of hydroxylated fullerene in the bioyogurt formulation makes it possible to reduce the fermentation process by 0.5-1.5 hours, improve the fermentation process and increase the number of lactic acid microorganisms in the finished product. According to the most significant indicator of taste and aroma, the experimental samples did not differ from the control and were typical for a high-quality finished product. The introduction of 0.08 % (II prototype) of water-soluble fullerene into the milk mixture promoted a slight release of whey during fermentation. The appearance of insignificant grains in the test samples of the finished product can be prevented by preparation (holding the fullerene in skim milk for 2-4 hours and subsequent homogenization). This will distribute the introduced substance in the finished product more evenly. It is recommended to introduce fullerene at the pasteurization stage in order to comply with the sanitary and hygienic conditions of production.