Usage of SNP-markers for identification of loci PL5 and PL8 controlling sunflower resistance to Plasmopara halstedii (Farl.) Berl. et de Toni

Downy mildew caused by an oomycete Plasmopara halstedii (Farl.) Berl. et de Toni is one of the most dangerous sunflower diseases. At the present moment, there have been found out 50 physiological races of the pathogen. The effective method to control the pathogen is introduction of dominant genes of resistance into the host-plant. The modern technologies of genotyping containing DNA-markers allowed controlling these genes presence at the each stage of breeding process. We developed 13 SNP-markers of genes Pl5 and Pl8 controlling resistance to 16 races and evaluated them on the lines-differentiators of sunflower resistance which are included into an international testing set for identification of P. halstedii races. We selected from them three SNP-markers 8R2, 5S2 and 5S3 which can be used for identification of the genes Pl5 and Pl8 controlling sunflower resistance to downy mildew pathogen.