Investigation of biologically active substances of dry extract from sea buckthorn leaves Hippophae rhamnoniedes

The aim of the study is to determine the content of biologically active substances in the dry extract ob-tained from the leaves of sea buckthorn Hippophae rhamnoides and to study the anti-inflammatory effect of the obtained extract. Objectives: to analyze the biological and toxicological purity of sea buckthorn leaves; to determine the concentration of water-soluble vitamins, tannins, flavonoids and the total level of antioxidants in the original plant material, dry extract and the drug Giporamin; to conduct a microbiological analysis of the purity of the obtained dry extract from the leaves of sea buckthorn; to study the anti-inflammatory effect of the extract obtained from the specified plant material when modeling formalin-induced edema in experimental animals. The results showed that sea buckthorn leaves meet the safety criteria: the content of heavy metals does not exceed the permissible limits, and there are no pathogenic microorganisms. The initial raw material contained 14.46 % tannins, 3.16 % flavonoids, 0.352 mg/g total antioxidants, and 0.08 mg/100 g vitamin C. The obtained dry extract contained 45.9 % tannins, 9.61 % flavonoids, 1.453 mg/g total antioxidants, and 0.34 mg/100 g vitamin C. Microbiological analysis confirmed that the extract complied with regulatory requirements. A study of anti-inflammatory activity using a forma-lin edema model in rats demonstrated a pronounced anti-inflammatory effect of the extract. The use of sea buckthorn leaf extract can be successful not only in medicine, but also in the food industry, for example, for enriching functional foods. This also indicates the importance of additional research to improve raw mate-rial processing methods and expand the areas of its use.