Quality of DNA for PCR extracted from sunflower with different methods

Studying of plants DNA with PCR method plays an important role in the agrarian activity. Preceding stage of DNA studying is its extraction from plant material. We studied quality of DNA extracted by the different methods from seeds, seedlings, and green leaves of sunflower. We used an inbred sunflower line as a research object. For analysis we used embryos, dry and presoaked during 24 h seeds, roots, 7-day seedlings, green leaves. DNA was extracted using five methods: 1 - a standard method of extraction (1% CTAB), 2 - a modified method (2% CTAB), 3-4 - extraction with sets (Diamond DNA Plant Kit D, Russia; Lumiprobe, Germany), 5 - extraction from green leaves (2% CTAB with absorbent carbon). Amplification was conducted in thermocycler S1000тм (BioRad, USA). Spectrophotometery was done at scanning spectrophotometer LEKI SS2110UV (Russia). Analyzing all used methods we concluded they allow extracting of DNA from dry seeds, seedlings and green leaves of sunflower with sufficient reliability and repeatability, it is proved by PCR results. We couldn't extract DNA from roots and presoaked seeds with the modified method (2% CTAB). The most economically profitable is first method (1% CTAB). Due to the results of spectrophotometery, the highest level of DNA clearance can be reached with method 3 - extraction by a set Diamond DNA Plant Kit D from seedlings and method 5 - extraction by 2% СТАВ with absorbent carbon from green leaves. The method 3 is more preferable by time necessary for DNA extraction.