The study of dihydroquercetin nanoemulsions derived by means of ultrasound

The purpose of the current investigation is to examine the possibility of using ultrasound treatment to obtain stable nanoemulsions of “oil-water” type to encapsulate biologically active substances of dihydroquercetin in order to increase and preserve its bioactive properties. Emulsions based on corn oil, distilled water and Gellan gum were obtained. The emphasis was placed on the study of the obtained emulsion stability in comparison with the reference sample stability. The morphological composition of the emulsions was investigated by confocal microscopy. The study also presents data on the assessment of the emulsions stability (% of the released oil fraction). The estimation of the peroxide number of freshly prepared emulsions and emulsions after 7 and 14 days of storage in aggressive conditions (at room temperature, with oxygen and ultraviolet supply). The results obtained in this study established the feasibility and effectiveness of the ultrasonic effect in the production of nanoemulsions, the morphology of which was more homogenous and finely dispersed, unlike the morphology of the reference sample, the agglomeration of particles was not observed. The stability of the emulsion obtained with the use of ultrasonic effect was 7 times higher than the values for the reference sample. The results of the evaluation of the peroxide number of emulsions proved the effectiveness of dihydroquercetin encapsulation approach to reveal the antioxidant properties. The results obtained The results showed that the use of ultrasonic effect in emulsions production can significantly increase the stability of emulsions while preserving the bioactive properties of dihydroquercetin. The values of the peroxide number of the emulsion obtained with the use of ultrasound slowed down the oxidizing processes by 1.7 times.