Study of the baromembrane technologies effect on the process of gelatine production

The aim of research is to study the operating conditions of ultrafiltration membranes effect on the concentration characteristics of gelatin solutions. Research objectives are to characterize the used ultrafiltration plant; to set operating parameters for ultrafiltration of gelatin broths; to investigate the influence of the ultrafiltration process on the physicochemical parameters of gelatin broth and its filtration products. Experimental studies were carried out on the basis of the Research Institute of Biotechnology at the Kemerovo State University (Kemerovo). The object of the study was gelatin broth. In experimental studies, a laboratory ultrafiltration unit MFU-R-45-300 (Russia) was used. The unit is equipped with three filtration devices equipped with ultrafiltration polymer filtration roll elements with pore sizes from 5 to 100 kDa, which makes it possible to obtain a protein concentrate with a high molecular weight. The controlled pressure was within 3.0 Bar, with a difference in the pressure and return manifolds of no more than 0.2-0.5 kgf/cm2. The protein content was determined on an analyzer of total nitrogen (protein) RAPID N ELEMENTAR, operating according to the Dumas method - combustion of a sample with registration of total nitrogen on a thermal conductivity detector. Determination of the content of dry substances was carried out on an IRF-454B2M refractometer. The sample was preliminarily diluted with water. The result was multiplied by the dilution factor. The concentration of hydrogen ions (pH value) was determined by the express method using a Testo 206-pH2 pH meter. The physicochemical parameters of the gelatin broth and the products obtained during the filtration (permeate and retentate) were determined. Ultrafiltration of the gelatin broth with polymer membranes made it possible to concentrate the solution in relation to the proteins of the control sample (the original gelatin broth) by 61.97 %, in relation to dry substances - by 62.88 %. The results obtained indicate the effectiveness of the use of baromembrane technologies, including ultrafiltration methods, in technological processes in the production of gelatin. It is proposed to use ultrafiltration units at the stage of concentration of gelatin broths in order to save resources and energy.