Study of the disintegrated membranes proteins of the pseudotuberculosis microbe antigenic properties

Disintegrated membranes of Yersinia pseudotuberculosis (DM Y. pseudotuberculosis) are antigenic promising drugs. The purpose of research is to study the protein and antigenic composition of the Y. pseudotuberculosis DM preparation. Using electrophoresis, the predominance of proteins with molecular weights of 23, 38, and 45 kDa was established in Y. pseudotuberculosis DM, among which the 38 kDa protein was present in the greatest amount. In mice immunized with Y. pseudotuberculosis DM, an increase in the respiratory activity of peritoneal macrophages and antibody genesis was noted. The rate of increase in the respiratory activity of peritoneal macrophages decreased at immunizing doses above 63 μg/mouse, and antibody formation also slowed down, which determined the value of the immunizing dose of 63 μg/mouse. When converted to a rabbit weighing 2.5 kg, the immunizing dose was 2 mg. We also carried out fivefold immunization of rabbits to obtain highly specific antibodies to Y. pseudotuberculosis DM. The antibody titers of the obtained serum in ELISA with whole Y. pseudotuberculosis cells were 1:25600, with Y. enterocolitica cells - 1:200, with cells of other representatives of the intestinal group of bacteria - 1:100-1:400. This indicates that species-specific proteins have the main antigenic activity in Y. pseudotuberculosis DM. The resulting antibodies interacted in immunoblotting with DM proteins having molecular weights of 38, 45, 58, 66 kDa. However, the most intense interaction was noted with a 45 kDa protein. During the psychrophilic cultivation of Yersinia, the molecular weights of the proteins found by us correspond only to the thermostable toxin and cell wall porins. These antigens are sufficiently resistant to low concentrations of sodium dodecyl sulfate and, together with lipopolysaccharide, can form the main antigenic composition of Y. pseudotuberculosis DM, which has species specificity.