Study of the biological, physical and chemical properties of the isolate of virus paragrip-3 cattle

This article presents the results of a study of the biological and physicochemical properties of bovine parainfluenza-3 virus (PG-3) isolate. It was found that after treatment with the 5-BDU preparation of all 3 tested strains, only a slight (0,25 lg) decrease in infectious activity was revealed. This circumstance testified to the fact that they are all RNA-containing viruses. When setting the hemagglutination reaction, the virus isolate as well as the reference and vaccine strains PG-3 agglutinated erythrocytes of guinea pig and cattle in a titer of 1: 8, as well as 1: 2 sheep and rabbit erythrocytes. Similar results were obtained in the hemadsorption reaction. All test strains of the virus diffusely adsorbed red blood cells of the above animals. When studying the physicochemical properties of the LD-9 isolate, it was found that it, as well as the reference and vaccine strains of the PG-3 cattle virus, is sensitive to the effects of ether, chloroform, T +560 C, pH 5.0 and 3.0.