Study of the chronic toxicity of the feed additive cobametine in laboratory animals

The purpose of this research was to study the effect of a new feed additive cobametine, which contains a chelated cobalt preparation based on butyric acid, on safety, dynamics of live body weight, hematological and biochemical parameters of white rats in a chronic experiment at doses of 153 mg/kg and 307.0 mg/kg. For this purpose, three groups of rats were formed: two experimental and one control, each of which consisted of ten individuals (five females and five males) aged 3-3.5 months with an average body weight of 228.33 g. The feed additive was administered to the animals to the first and second experimental groups daily through a special probe once inside the stomach for 90 days. The third group of rats served as a biological control and received distilled water. When studying the chronic toxicity of cobametine, it was found that with long-term administration of the additive in experimental groups, the safety of laboratory animals decreases by 10 % relative to the control group. At the end of the experiment, the total gain during the experiment in the control group was 76.55 g, which is 17.01 % lower than the 1st experimental group, but 13.53% higher than the 2nd experimental group. In the biochemical analysis of blood serum and general blood analysis, the difference between the animals of the experimental and control groups in the content of the analyzed indicators was insignificant; their level corresponded to the parameters of physiological indicators for this type of animal. The results obtained should be taken into account when compiling a complete picture of the chronic toxic effect of the additive, taking into account pathological and histological studies in order to predict possible side effects when choosing doses in clinical trials.