Express-evaluation of oleic and linoleic fatty acids content in oil in milled sunflower kernels by means of IR-spectrometry

The purpose of the work is to create a method for determination of the basic fatty acids in oil in milled sunflower kernels by means of the IR-spectrometry. We conducted our researches in the laboratory of biochemistry with sunflower seeds produced in 20152018 on the fields of the All-Russian Research Institute of Oil Crops by Pustovoit V.S. (Krasnodar) and its branches (Krasnodar region). We determined a set of fatty acids in milled kernels due to the State Standards R 51483-99 and R 51486-99 using a gas chromatograph “Chromatec-Kristall 5000” equipped by automatic dispenser on a capillary column SolGelWax 30 m х 0.25 mm х 0.5 mcm in a gaseous fluid current - helium bearer with a speed 25 cm per sec, with a programmed temperature within 185-235 °С. Prepared kernel samples were milled and we registered the obtained spectra in a spec trum 3500-12500 cm-1 with resolution 16 cm-1, according to the manual for the IR-spectrometer MA-TRIX-I, produced Bruker Optics (Germany)...