Quick-assay of the content of the main fatty acids in oil of rapeseed seeds by means of IR-spectrometry

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The purpose of this work was development of calibrating models for IR-analyzer MATRIX-I allowing to do the quick-assay of contents of oleic, linoleic and linolenic acids in oil of rapeseed seeds. The researches were conducted on seeds of winter and spring rape-seed of 2014-2015 yields at the laboratory of biochemistry of VNIIMK. A chromatographic analysis was done on a gas chromatograph “Chromatech- Kristall 5000” with an automatic batcher DAG-2M on a capillary column SolGelWax 30 m x 0,25 mm x 0,5 mkm in a floor of carrier-gas helium with a speed 22 cm/m and with a temperature programming within 178-230 оС. Creation of methyl ethers and their chromatography was conducted due to the State Standards R 51486-99 (A creation of methyl ethers of fatty acids) and R 51483-99 (A definition of a weight percentage of methyl ethers of individual fatty acids to their sums by means of a gas chromatography). Spectra were registered subject to a manual on OS OPUS in a spectral range 3500-12500 cm -1 with a definition 16 cm -1 on IR-analyzer MATRIX-I by Bruker Optics (Germany). The rates of oleic, linoleic and linolenic acids in oil of seeds of erucic acid free rapeseed are from 92.0 to 93.5% of the total sum of all 13 fatty acids, so they are the primary fatty acids for rapeseed. To do a calibration of IR-analyzer a lot containing 68 rapeseed seed sample were used; the seeds had a maximally possible variation range on contents of oleic acid in oil - 61.7-82.7%. A rest content of erucic acid in all samples did not exceed 0.03% of the total sum of fatty acids. The usage in a calibrating lot of rapeseed samples with erucic acid content 10.0 and 16.9% significantly impaired a calibrating dependence so they were eliminated. An error in determination of oleic, linoleic and linolenic acids contents obtained on the calibrating models by means of IR-spectrometry does not exceed substantively the standard method. The calibrating models for determination of contents of the primary fatty acids in oil of rapeseed seeds in a small test charge in a glass with diameter 22 mm were developed. It allows swiftly conducting preliminary estimation of breeding material with a high speed, more than 100 samples during working shift, and to control fatty-acid composition of separate plants.

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Rapeseed, seeds, fatty-acid composition of oil, ir-spectrometry, calibrating model

Короткий адрес: https://sciup.org/142151273

IDR: 142151273

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