Elements of the laboratory regulations for the production of microbiologics in a “wettable powder” preparative form based on antagonistic fungi of the genus Trichoderma during surface cultivation on liquid nutrient media

There were developed the elements of the laboratory regimen for production of microbiopreparation in the form of ‘wettable powder' on surface cultivation on liquid nutrient mediums of perspective fungus strain-producer Т-2 Trichoderma sp. from a collection of the laboratory of biomethods. Period of 10 days was determined as optimal for surface cultivation on a liquid nutrient medium of Rudakova. Optimal ratio between volumes of culture and nutrient medium was 2.0%. Test samples of microbiopreparation in the form of ‘wettable powder' obtained on surface cultivation of the strain Т-2 Trichoderma sp. on a liquid nutrient medium formed a stable suspension in water and had an initial titer 109-10 CFU/g and a moisture content of 5.4-6.5 %. When storing a ‘wettable powder' microbiopreparation in packages with air access and in jars with a tightly closed lid, regardless of the method of biomass separation, under conditions of permanent low temperature (-18 °С), the titer have not decreased within twelve months. When storing under variable temperature (+24…+26 °С), after six months the titer of a test sample of a ‘wettable powder' microbiopreparation decreased by one order of magnitude both in a jar, and in a package and remained the same for twelve months (1.2 × 108 CFU). Under permanent high temperature (+30 °С) the titer decreased by four orders of magnitude to 105 CFU/g in twelve months. It is not reasonable to add antiaging agents to keep test samples of microbiopreparation in the form of ‘wettable powder' based on a fungus Т-2 Trichoderma sp. with insignificant decrease of the titer (from 109 to 108 CFU/g) during twelve months under variable temperature.