To the ultrastructural organization of epitheliocytes of exocrine glands

Pieces of the sublingual multi-duct salivary glands of a domestic sexually mature large white pig were fixed in a 4 % paraform solution in 0.1 M phosphate buffer (pH = 7.4) with additional fixation in a 1 % OsO4 solution, dehydrated in alcohols of increasing concentration. Taking into account the recommendations of G. Gayer, pieces of organs were poured into araldite according to the standard technique. Contrasting was performed according to Reynolds. In the cytoplasm of the mucocytes of the sublingual multi-duct salivary gland, the agranular endoplasmic reticulum predominates, which gives oxyphilic staining. According to electron microscopic studies of mucous glandulocytes in the acini of the sublingual salivary glands of domestic pigs, the secretory vacuoles of the cytoplasm are large, with pronounced electron-dense membranes. The content of vacuoles in mucocytes of one acinus is different. In the mucous cells of the sublingual salivary glands, the vacuolization of the hyaloplasm is small. In the apical part of the cytoplasm, the cytolemma has vacuoles of various sizes, indicating a merocrine type of secretion of mucous cells. Of the organelles, the most developed are the agranular reticulum and the lamellar complex. The nuclei of mucocytes of basal adherence, hyperchromic, with unexpressed nucleoli. The ultrastructural organization of the sublingual multi-duct salivary glands of a sexually mature domestic pig has its own morphological features in the structure of terminal glandulocytes and epithelial cells of the intralobular and interlobular excretory systems. Knowledge of the ultrastructure of the glandular epithelium makes it possible to identify early morphological changes in the parenchyma of the exocrine glands during the development of various pathologies.