Classification of national variety accessions of cabbage Brassica oleracea L. with the use of SSR markers

It is important to reveal the genetic base of breeding genetic material used for development of new breeding accessions among diverse Brassica oleracea L. (CC, 2n = 18). Traditional varieties, hybrids and new ones recently developed are the main genetic resources. Classification of a collection with DNA markers enables to reveal valuable genotypes and establish the breeding accession pedigree that allows developing the new accessions with sustainable economically valuable traits. The use of microsatellite markers (SSR) in B. oleracea L. has shown high efficiency in discovering genetic polymorphism between varieties and within varieties as well. In this study, 16 primer pairs have been taken to amplify microsatellite loci of genomic DNA in national 24 breeding accessions of cabbage. On the basis of the data obtained the dendrogram has been constructed with use of Jaccard's coefficient. All loci studied were high informative, where 14 out of 16 had a PIC > 0.5. As a result, the level of genetic polymorphism has reached 85...