Clinical significance of MMP-1 and TIMP-1 levels in follicular fluid at various outcomes of auxiliary reproductive technologies

Objective. The purpose of the paper is to determine the clinical significance of MMP-1 and TIMP-1 in follicular fluid (FF) and the role of MMP-1/TIMP-1 system in ovarian regulating function. Analysis of MMP-1 and TIMP-1 enzymes, which have a pro-inflammatory effect, cause degradation of the extracellular matrix and limit collagen breakdown, can explain the lack of follicle growth, development and ovulation in assisted reproductive technology (ART). Materials and Methods. The authors examined matrix metalloproteinase (MMP-1) and tissue inhibitor (TIMP-1) in FF in 20 patients with various infertility origins who underwent fertility treatment (ART). To analyze MMP-1/TIMP-1 system, a quantitative solid-phase enzyme-linked immunosorbent assay of a “sandwich” type was used. FF was taken with follicle puncture in ART programs. An automatic photometer Multiskan FC (USA, 2012) was used for immunoenzymatic analysis. Results. MMP-1 and TIMP-1 concentration in FF depends on the infertility origin of each particular patient. MMP-1 concentration was shown to increase in patients with tubal peritoneal infertility. In ovarian insufficiency, low levels of estrogens and progesterone were accompanied by a decrease in MMP-1 concentration and an increase in TIMP-1 activity. Such changes in metalloproteinase levels can lead to increased processes of ovarian tissue fibrosis and sclerosis, development of follicular atresia and increased risk of ovarian failure.