Cultural traits and growth of a promising fungal strain M-24 Penicillium sp. - an antagonist of phoma pathogen on sunflower on liquid nutrient media

A microbiological method decreasing harmfulness of Phoma rot on sunflower, which is one of the prevailing disease in all regions of crop cultivation, is developed at the V.S. Pustovoit All-Russian Research Institute of Oil Crops. The cultural traits of antagonist fungus of phoma pathogen on sunflower M-24 Penicillium sp. on three agar media and strain growth on four complicated liquid nutrient media were studied at surface cultivation of a strain-producer to develop elements of technological regimen for production of microbiopreparations in a preparative form wettable powder. On the tenth days of cultivation at +25 °С temperature, strong growth of the fungus with abundant conidial sporulation and maximal diameter of a colony was noted on the agar medium by Rudakov. The medium by Rudakov is stated as optimal among complicated liquid nutrient media. In ten days of the surface cultivation, a thick folded mycelial film with an abundant sporulation was formed on this medium as well as maximal weight of a dry mycelium - 1.44 g per 100 ml of nutrient medium vs. 0.20-1.39 g on the other media.