Metabolism and chemiluminescent activity of neutrophilic granulocytes in patients with different sensitivity to acetylsalicylic acid in acute coronary syndrome

Objective: to study the features of the chemiluminescent state and the activity of NAD (P) -depen- dent dehydrogenases in neutrophilic granulocytes of blood in patients with different sensitivity to acetylsalicylic acid (ACA) in acute coronary syndrome (ACS).Materials and methods: The study included 53 patients with ACS. Evaluation of resistance or sensitivity to ASA was performed in vitro by incubating platelet-rich plasma with adenosine diphosphate and ASA to determine the level of aggregation. The state of respiratory explosion of neutrophils was investigated by the method of chemiluminescence. The activity of enzymes in neutrophils was studied by the bioluminescent method.Results of the study: In patients with ACS-resistant ACS, the rate of synthesis of primary and secondary active forms of oxygen was reduced, and the index of luminol-dependent activation of neutrophils was reduced. The intensity of substrate stimulation of glycolysis and oxidation of glucose by the pentose phosphate pathway is increased.Conclusion: With resistance to ASA in patients with ACS, there are abnormalities in the metabolism and functional activity of neutrophils, which is of interest in studying the intercellular relationships of thrombus formation.