Microsatellite loci as markers for identification and sertification of sunflower lines and hybrids of VNIIMK breeding

17 inbred lines and two hybrid combinations of VNIIMK breeding were analyzed on 10 known microsatellite loci. The researches resulted in 10 microsatellite loci only nine were polymorphic. Average amount of alleles per locus was 2.2. Molecular-genetic passports were made and originality of each inbred line and hybrid was determined by means of polymorphic loci. Hybridological analysis showed that two loci from nine - Ha 432 and Ha 1442-1 - had dominant type of inheritance. The seven ones had codominant type of inheritance; it allows to establish hybrid level in line crosses. It was established that using of studying microsatellite loci for identification and certification of sunflower lines and hybrids combinations developed in VNIIMK is advisable. Cluster analyses and dendrogramm were made on the base of the results of 17 inbred lines passportisation on nine microsatellite loci. At a level of similarity between lines from 0.19 to 0.97 the originality of each line and hybrid combination is revealed. Their molecular-genetic passports on the base of nine loci are made. The suitability of nine loci for genetic purity tests of sunflower seeds in commercial lots is shown. Seven codominant loci are suitable for definition of a "hybridity range".