Molecular genetic analysis of Pinus sylvestris L. and Pinus sibirica du Tour populations in Perm krai based on polymorphism ISSR-PCR markers

DNA polymorphism has been studied, indicators of genetic diversity and genetic structure of 3 populations of Pinus sylvestris L. and 3 populations of Pinus sibirica Du Tour in the Perm Krai have been determined. In the populations of P. sibirica , 102 ISSR-PCR markers were found, of which 88 were polymorphic ( P95 = 0.863), and in the populations of P. sylvestris - 113 ISSR-PCR markers, 100 of which were polymorphic ( P95 = 0.885). The populations of the two studied species of woody plants are characterized by high genetic diversity. At the same time, in P. sibirica , the indices of genetic diversity were slightly higher ( HE = 0.195; ne = 1.335; na = 1.330) than in P. sylvestris ( HE = 0.166; ne = 1.268; na = 1.212). The analysis of the genetic structure showed that the coefficient of genetic subdivision ( GST ) in the two studied species of the genus Pinus are similar and amount to 0.320 in P. sibirica and 0.303 in P. sylvestris . The populations of Siberian pine and Scots pine are characterized by an average degree of genetic differentiation, since the interpopulation component accounts for 32.0% and 30.3% of the genetic diversity of these species, respectively. Using the Mantel test, a high correlation was found between genetic and geographical distances in P. sibirica populations (R2 = 0.6871), while P. sylvestris showed a low correlation (R2 = 0.0649). The data obtained are relevant for the preservation of the gene pools of the studied two species of the genus Pinus in the Perm Krai.