Moleculargenetic diversity of the collection of 14 sunflower lines - donors of resistance to broomrape race G based on isoenzymes and SSR-loci

Sunflower is main oil crop in the Russian Federation. One of the general factors decreasing its yields is broomrape. The most effective method to protect the crops is breeding of resistant forms of sunflower. The purpose of this work is to genotype and to analyze basing on isoenzymes and SSR-loci the molecular-generic diversity of the collection of lines -donors of resistance to broomrape race G bred at V.S. Pustovoit All-Russian Research Institute of Oil Crops. An object of the research was 14 lines of the different origin - donors of the resistance to this broomrape race: 2RGB, 2RGNV, 2RGA, 2RGN, 2RGS, RGL1, RGL2, RGP1, RGP2, RGB, RGN, RGM, RGA, and RG. DNA was isolated by STAB-method from the cotyledons of seven-day etiolated sunflower seedlings. Genotyping was conducted using 15 microsatellite loci and four isoenzymes systems. The indicators of a discriminating potential of used 15 microsatellite loci were calculated by the standard formulas. Cluster analysis was done by Ward-method in a program Statistica 6.0. There were identified 14 lines-donors. The collection was analyzed by the isoenzymes and microsatellite loci; the unicity is 64% and 100%, respectively. The discriminating potential of a system of DNA-markers is average. Due to cluster analysis, all lines were divided into two groups. The maximal distance between the lines was 7.4. In spite of the studied lines have a common trait - resistance to the broomrape race G, their molecular traits distinguished. According to the different genetic background, these lines can be used as a source of the additional diversity when involving into the breeding of sunflower resistant to broomrape race G.