Morphological changes in wild boar testile parenchma with chlamydiosis

A distinctive feature of chlamydia is the often-asymptomatic course of the disease, and without timely, high-quality diagnosis, the pathogen can persist in the body for many months and even years, causing serious diseases and spreading unhindered among animals. The purpose of research is to study the morphological changes in the parenchyma of the testes of wild boar with chlamydia. The objects of the study are the testes of wild boars aged 10-11 months. Histological sections were cut on an MZP-01 technomy microtome with a thickness of 5 microns. The finished histological preparations were stained with hematoxylin-eosin, according to Van Gieson and Pavlovsky. Using microscopy of histological preparations, the structure of the testes and changes in them were studied. Microscopic examinations were carried out with a Micros microscope at a magnification of 200-400 times in 10 fields of view of correctly oriented sections. At least 100 cells were studied. The established numerical data were subjected to variable statistical processing according to Student using MS Excel 2010. When analyzing morphometric studies of the spermatogenic epithelium of the convoluted seminiferous tubules of the testes, it was noted that in the convoluted tubules that retained their structure, the thickness of the spermatogenic epithelium was 14.19 ± 0.23 μm, the number of cells Sertoli are smaller than spermatogonium and spermatocytes. As a result of the studies, we can conclude that in sick animals, already at a young age, the parenchyma is affected with the development of alterative processes, in which there is a decrease in spermatogenesis, which negatively affects the function of the testes, and mechanical transmission of infectious agents during mating is possible.