Morphological changes of field isolates of the genus Trichoderma to herbicides

The aim of the research is to study the morphological changes of fungi of the genus Trichoderma when using glyphosate at intervals of 24, 48, 96 hours. Isolated isolates of T.koningii, T.veride, T.harzianum, T.asperellum diluted in an aqueous extract at a dilution of 106 were extracted in a 10 % preparation of glyphor (the active ingredient is glyphosate). After each 24, 48, 96-hour exposure, control seeding was carried out on agarized Czapek media. After incubation at a temperature of 23 0C, visual inspection and microscopy of fungal colonies were carried out, taking into account the color, structure and consistency of conidia. The fungus of the genus T. koningii was more susceptible to chemical action, which was manifested by a change in mycelium growth, characteristic ragged conidiophores, and a decrease in the number of acospores on Czapek's nutrient medium. T.asperellum under the influence of the herbicide after 48 hours changed the shape of the conidia, the walls of the conidiophores decreased, upon visual examination after 48-96 hours the color of the fungus colonies was transparent, slightly whitish. The isolate of T.harzianum proved to be the most resistant to glyphosate, which was capable of rapid mycelium recovery after 48 h. Thus, with the combined use of biological products based on fungi of the genus Trichoderma and herbicides based on glyphosate, the action of the former can be slowed down, which should be taken into account in their development and application.