Multiplex multilocus real time PCR for analysis and control of avian leukosis virus subgroups A, B, J and K in Russia

The avian leukosis virus (ALV) belongs to genus Alpharetrovirus of Retroviridae family and has a diploid genome consisting of a single-stranded RNA. ALV subgroups A, B, C, D, E, J and K are specific for chicken. The classification is based on differences in the viral coat protein structure. ALV, in particular ALV subgroup J, causes huge damage to industrial poultry. The gold standard for detecting ALV is virus isolation in CEFs or DF-1 cell cultures. This method has significant disadvantages, i.e. it takes 7-9 days, requires specialized facilities and equipment. Enzyme-linked immunosorbent assay based on detection of the ALV p27 group-specific antigen is the most widely used, but it also has significant deficiencies, the main of which are false positive results due to the expression of p27 by endogenous viruses and lack of sensitivity. In this study, a test system has been developed to detect exogenous viruses of the most common ALV subgroups A, B, J, and K and to control the spread of ALV...