Multiplex PCR for indication and differentiation of cables of respiratory viral infections of cattle

Thus, as a result of the conducted polymerase chain reaction, it has been established that the mixture of oligonucleotide primers when interacting with one of the DNA markers does not interfere with accumulation of specific amplification products. Each biological pathogen corresponds an individual fluorescent marker for bovine viral diarrhea channel R6G, for parainfluenza-3-channel Fam, and for infectious rhinotracheitis Cy5.