Neuroimmune parameters in adult recipients after the repeated transplantation of immune cells with different functional characteristics which was held in the juvenile development period

Background. The main regulatory systems of the body immune and nervous functioning in close interaction play a crucial role in maintaining homeostasis at all stages of ontogenesis; and the nature of their interaction determines the characteristics of the psychophysiological status of individuals and its reserve capabilities. Earlier, we demonstrated the possibility of a directed change in the parameters of the functional activity of these systems in sexually mature animals by transplantation of immune cells with certain functional characteristics. The aim of the study was to investigate the neuroimmune parameters in adult recipients which repeated transplantation of immune cells with different functional parameters, peculiar animals with opposing types of behavior, was performed in the juvenile development period. Methods: Studies were carried out on male (CBAxC57BL/6) F1 mice, starting from 4-5-week age, 3 times transplantation of immune cell with certain functional characteristics was conducted. Phenotyping of transplanted cells and splenocytes of recipients was carried out by flow cytofluorometry with monoclonal antibodies against CD3+, CD4+, CD8+, CD19+, CD14+, CD115+, labeled by fluorochromes with different emission spectra. In syngeneic recipients in the 12 -week age parameters of the exploratory behavior, proliferative activity of splenocytes, and the intensity of the humoral and cellular immune response were also evaluated. Results: Mice (CBAxC57BL/6) F1, subjected to the juvenile period of development of a 3-fold transplantation of immune cells from syngeneic donors with opposing types of behavior, were characterized by a different neuroimmune status in the sexually mature age, that was expressed in the formation of a certain predominant stereotype of behavior, different intensity of the humoral and cellular links of the immune response, differences in the phenotypic characteristics and proliferative activity of immune cells. Conclusion: In animals that grew under conditions of multiple transplantation of immune system cells with various functional characteristics, a certain nature of neuroimmune interaction was formed, which provided various adaptive capabilities of recipients.